Subtomogram average of flagellar axoneme doublet from the wild type Tetrahymena thermophila
Fabritius AS, Bayless BA, Li S, Stoddard D, Heydeck W, Ebmeier CC, Anderson L, Gunnels T, Nachiappan C, Whittall JB, Old W, Agard DA, Nicastro D, Winey M
Mol Biol Cell (2021) 32 pp. br8-br8 [ Pubmed: 34406789 DOI: doi:10.1091/mbc.E20-12-0786 ]
Fabritius AS, Bayless BA, Li S, Stoddard D, Heydeck W, Ebmeier CC, Anderson L, Gunnels T, Nachiappan C, Whittall JB, Old W, Agard DA, Nicastro D, Winey M
Mol Biol Cell (2021) 32 pp. br8-br8 [ Pubmed: 34406789 DOI: doi:10.1091/mbc.E20-12-0786 ]
Cryo-EM reconstruction of VP5*/VP8* assembly from rhesus rotavirus particles - Upright conformation
Resolution: 3.4 Å
EM Method: Single-particle
Fitted PDBs: 6wxe
Q-score: 0.472
Herrmann T, Torres R, Salgado EN, Berciu C, Stoddard D, Nicastro D, Jenni S, Harrison SC
Nature (2021) 590 pp. 666-670 [ DOI: doi:10.1038/s41586-020-03124-4 Pubmed: 33442061 ]
- Calcium ion (40 Da, Ligand)
- Rotavirus vp4, vp6, vp7 assembly in the spike conformation (1 MDa, Complex from Rotavirus A (strain RVA/Monkey/United States/RRV/1975/G3P5B[3]))
- Outer capsid protein vp4 (86 kDa, Protein from Rotavirus A (strain RVA/Monkey/United States/RRV/1975/G3P5B[3]))
- Intermediate capsid protein vp6 (44 kDa, Protein from RV-A)
- Outer capsid glycoprotein vp7 (37 kDa, Protein from Rotavirus A (strain RVA/Monkey/United States/RRV/1975/G3P5B[3]))
- 2-acetamido-2-deoxy-beta-d-glucopyranose (221 Da, Ligand)
Resolution: 4.3 Å
EM Method: Single-particle
Fitted PDBs: 6wxf
Q-score: 0.306
Herrmann T, Torres R, Salgado EN, Berciu C, Stoddard D, Nicastro D, Jenni S, Harrison SC
Nature (2021) 590 pp. 666-670 [ DOI: doi:10.1038/s41586-020-03124-4 Pubmed: 33442061 ]
- Calcium ion (40 Da, Ligand)
- Intermediate capsid protein vp6 (44 kDa, Protein from RV-A)
- Outer capsid protein vp4 (86 kDa, Protein from Rotavirus A (strain RVA/Monkey/United States/RRV/1975/G3P5B[3]))
- Outer capsid glycoprotein vp7 (37 kDa, Protein from Rotavirus A (strain RVA/Monkey/United States/RRV/1975/G3P5B[3]))
- Rotavirus vp4, vp6, vp7 assembly in an intermediate confromattion (1 MDa, Complex from Rotavirus A (strain RVA/Monkey/United States/RRV/1975/G3P5B[3]))
- 2-acetamido-2-deoxy-beta-d-glucopyranose (221 Da, Ligand)
Cryo-EM reconstruction of VP5*/VP8* assembly from rhesus rotavirus particles - Reversed conformation
Resolution: 3.3 Å
EM Method: Single-particle
Fitted PDBs: 6wxg
Q-score: 0.509
Herrmann T, Torres R, Salgado EN, Berciu C, Stoddard D, Nicastro D, Jenni S, Harrison SC
Nature (2021) 590 pp. 666-670 [ DOI: doi:10.1038/s41586-020-03124-4 Pubmed: 33442061 ]
- Rotavirus vp4, vp6, vp7 assembly in the foldback conformation (1 MDa, Complex from Rotavirus A (strain RVA/Monkey/United States/RRV/1975/G3P5B[3]))
- Calcium ion (40 Da, Ligand)
- Intermediate capsid protein vp6 (44 kDa, Protein from Rotavirus A (strain RVA/Monkey/United States/RRV/1975/G3P5B[3]))
- Outer capsid protein vp4 (86 kDa, Protein from Rotavirus A (strain RVA/Monkey/United States/RRV/1975/G3P5B[3]))
- Outer capsid glycoprotein vp7 (37 kDa, Protein from Rotavirus A (strain RVA/Monkey/United States/RRV/1975/G3P5B[3]))
- 2-acetamido-2-deoxy-beta-d-glucopyranose (221 Da, Ligand)
Stoddard D, Zhao Y, Bayless BA, Gui L, Louka P, Dave D, Suryawanshi S, Tomasi RF, Dupuis-Williams P, Baroud CN, Gaertig J, Winey M, Nicastro D
Mol Biol Cell (2018) 29 pp. 2566-2577 [ DOI: doi:10.1091/mbc.E18-06-0405 Pubmed: 30133348 ]
Stoddard D, Zhao Y, Bayless BA, Gui L, Louka P, Dave D, Suryawanshi S, Tomasi RF, Dupuis-Williams P, Baroud CN, Gaertig J, Winey M, Nicastro D
Mol Biol Cell (2018) 29 pp. 2566-2577 [ DOI: doi:10.1091/mbc.E18-06-0405 Pubmed: 30133348 ]
Stoddard D, Zhao Y, Bayless BA, Gui L, Louka P, Dave D, Suryawanshi S, Tomasi RF, Dupuis-Williams P, Baroud CN, Gaertig J, Winey M, Nicastro D
Mol Biol Cell (2018) 29 pp. 2566-2577 [ DOI: doi:10.1091/mbc.E18-06-0405 Pubmed: 30133348 ]
Stoddard D, Zhao Y, Bayless BA, Gui L, Louka P, Dave D, Suryawanshi S, Tomasi RF, Dupuis-Williams P, Baroud CN, Gaertig J, Winey M, Nicastro D
Mol Biol Cell (2018) 29 pp. 2566-2577 [ DOI: doi:10.1091/mbc.E18-06-0405 Pubmed: 30133348 ]